Figure 2

BMAL1/REV-ERBα Circadian Rhythmic Loop Disruption was observed in adipose tissue of HF. (A–C) Protein expression of BMAL1 and REV-ERBα measured by Western blot in WAT. (D–F) mRNA expression of Clock, Bmal1 and Rev-erbα measured by RT‒qPCR in WAT. (G–I) Protein expression of BMAL1 and REV-ERBα measured by Western blot in BAT. (J–L) mRNA expression of Clock, Bmal1 and Rev-erbα measured by RT‒qPCR in BAT. Data are mean ± SD, n = 6. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.