Figure 5 | Scientific Reports

Figure 5

From: Excessive fat expenditure in MCT-induced heart failure rats is associated with BMAL1/REV-ERBα circadian rhythmic loop disruption

Figure 5

Disruption of the BMAL1/REV-ERBα circadian rhythmic loop resulted in lipolysis and beiging of WAT and reducing lipid storage in HF. (A,C–F) Protein expression of ATGL, HSL, PKA and p-PKA measured by Western blot in WAT. (B,G) Immunostaining and positive areas of UCP-1 in WAT. (H-J) mRNA expression of Hsl, Atgl and Peripilin in WAT. (K,L) mRNA expression of FFA β-oxidation genes, including Cpt1 and acyl-CoA. (M–P) mRNA expression of lipogenic genes, including Scd1, Fas, Mgat and Dgat2. (Q–T) mRNA expression of beige adipocyte markers, including Cd137, Tbx-1, Ucp-1 and Zic-1. Data are mean ± SD, n = 6. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

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