Figure 4
Western blotting to detect the expression of SHMT1 and SHMT2 in clinical samples. (A and E): Western blot to determine the levels of SHMT1 and SHMT2 expression in clinical BLCA tissue specimens; (B and F): Statistical analysis of the data using ImageJ for the band’s grey scale values and GraphPad Prism version 8 for the histogram, with unpaired t-test (n = 3), * P < 0.05, ** P < 0.01, *** P < 0.001. (C and G): Immunohistochemical detection of SHMT1 and SHMT2 expression levels in clinical BLCA tissue specimens; (D and H): ImageJ analysis of photographs to determine the target gene expression; GraphPad Prism version 8 for the histogram; statistical analysis using unpaired t-test (n = 3), * P < 0.05, ** P < 0.01, *** P < 0.001.
