Figure 5
From: The 3D in vitro Adrenoid cell model recapitulates the complexity of the adrenal gland
Fluorescence confocal microscopy analysis of spatial distribution of MTT and H295R cells inside the growing Adrenoid. Representative images of the Adrenoid at different time points of growth acquired by confocal microscopy. MTT and H295R cells were prestained with fluorescent viability staining (green and red, respectively) before generation of Adrenoids. Confocal images were taken through an HC PL 10x/0.30NA objective using sequential acquisitions of the green and red channels. Images are shown as the maximum intensity projection along the z-axis. The same Adrenoid was followed at 12 h from induction before transfer into chamber slide, and at T0 (48 h from induction) and at day 3, 5, 10 days after. The H295R cells tend to form clusters homogeneously diffused in an MTT core even before the formation of the compact 3D structure. From these images it is clear that Adrenoid growth is mainly due to MTT proliferation. Scale bars are indicated at the right bottom.
