Figure 6

Z-axis distribution of H295R clusters and MTT cells inside the Adrenoid. (A) Different z-focal planes acquired by confocal microscopy in the lower hemisphere of the same Adrenoid at day 5 to better explore H295R cluster spatial distribution inside of the Adrenoid core. At the bottom of the Adrenoid most of the cells were MTT (prestained in green fluorescence) and very few, mostly single, H295R cells (prestained in red fluorescence) are appreciated (a, planes 1–4). Proceeding towards the central part of the Adrenoid (from plane 5–15) H295R cells tended to be organized in clusters, which are homogeneously distributed both in the centre and at the periphery of the Adrenoid (b). The homogenous distribution of H295R clusters was better appreciated in the central confocal sections of the Adrenoid (16–24 stack-planes) (c). Scale bars are indicated at the right bottom.