Figure 2

Linear regressions of different data parameters showing correlation between number of root tips and normalised cumulative total compound signal (a) and dry root weight in mg and normalised cumulative total compound signal (b). Data was restricted to samples of non-split root BK (BK) and split root BK isolated compartment (A) of BK grown with no neighbour (BK-0), BK with a same species neighbour (BK-BK), and BK with a P neighbour (BK-P) (n = 3–5/condition). Data was normalised first through use of internal standard (3,5-Di-tert-butyl-4-hydroxybenzoic acid) and pooled quality control samples with the LOWESS method in MS DIAL. Total compound signal (TCS) was calculated by annotating adducts and then combining adduct and non-adduct signals of the same compound. All signals were added together within a single sample to create a cumulative value to represent total metabolite signal. Positive and negative ionisation mode data is combined in these figures.