Table 1 List of bacterial strains used in this study.

From: Cobalt complexes modulate plasmid conjugation in Escherichia coli and Klebsiella pneumoniae

Species

Features

Source of strain

Escherichia coli

NCTC 10418/ATCC 10536 susceptible reference strain

NCTC

Staphylococcus aureus

NCTC 12981/ATCC 25923 susceptible reference strain, weak β-lactamase producer

NCTC

Escherichia coli

Escherichia coli J53 with the conjugative pKM101 plasmid, which confers resistance to β-lactams

DSMZ GmbH

Escherichia coli

Escherichia coli J53 with the conjugative R388 plasmid, which confers resistance to trimethoprim and sulphonamides

DSMZ GmbH

Escherichia coli

Escherichia coli J53 with the conjugative R6K plasmid, which confers resistance to β-lactams and streptomycin

DSMZ GmbH

Escherichia coli

Escherichia coli J53 carrying the conjugative RP4 plasmid, which confers resistance to β-lactams, kanamycin, and tetracycline

DSMZ GmbH

Escherichia coli

Escherichia coli J53 with the hygromycin resistance gene hph inserted into the attTn7 site (HygR)

This study

Klebsiella pneumoniae

Klebsiella pneumoniae Ecl8 with mCherry-aph inserted into chromosomal putPA intergenic region. Resistant to kanamycin and expresses mCherry constitutively

30

Klebsiella pneumoniae

Klebsiella pneumoniae Ecl8 carrying pKpQIL with gfp-aph inserted into the blaKPC gene. Resistant to kanamycin and expresses GFP constitutively

30

Escherichia coli

Escherichia coli ST131 EC958c with mCherry-aph inserted into chromosomal putPA intergenic region. Resistant to kanamycin and expresses mCherry constitutively

30

Escherichia coli

Escherichia coli ST131 EC958c carrying pCT with gfp-aph inserted into the blaCTX-M-14 gene. Resistant to kanamycin and expresses GFP constitutively

30

  1. HygR, hygromycin resistant. DSMZ GmbH strains were obtained from the German Collection of Microorganisms and Cell Cultures, and NCTC strains were obtained from the National Collection of Type Cultures.