Table 3 Conjugation frequency data for RP4, R6K, R388 and pKM101 plasmids in liquid broth and solid agar mating in the presence of 100 µg/mL cobalt compounds or DMSO vehicle control.

From: Cobalt complexes modulate plasmid conjugation in Escherichia coli and Klebsiella pneumoniae

Compound

Conjugation frequency

RP4

R6K

R388

pKM101

Solid

Liquid

Solid

Liquid

Solid

Liquid

Solid

Liquid

DMSO

4.26 × 10–3 ± 1.10 × 10–3

4.26 × 10–3 ± 1.10 × 10–3

1.19 × 10–3 ± 4.59 × 10–4

6.26 × 10–5 ± 3.62 × 10–5

4.19 × 10–2 ± 5.3 × 10–3

2.11 × 10–3 ± 1.02 × 10–3

4.33 × 10–1 ± 2.93 × 10–1

1.06 × 10–2 ± 2.44 × 10–3

Co4

6.79 × 10–2 ± 1.08 × 10–2

9.06 × 10–3 ± 7.39 × 10–4

2.95 × 10–5 ± 2.56 × 10–5

1.58 × 10–5 ± 7.40 × 10–6

1.82 × 10–2 ± 6.85 × 10–3

5.63 × 10–4 ± 2.66 × 10–4

3.99 × 10–1 ± 4.73 × 10–1

1.08 × 10–2 ± 1.24 × 10–3

Co5

4.50 × 10–2 ± 3.85 × 10–2

1.11 × 10–2 ± 1.57 × 10–3

6.52 × 10–4 ± 4.99 × 10–4

6.53 × 10–5 ± 2.12 × 10–5

6.23 × 10–2 ± 4.02 × 10–2

1.33 × 10–3 ± 6.26 × 10–4

4.97 × 10–1 ± 3.96 × 10–1

1.14 × 10–2 ± 1.54 × 10–3

Co6

1.62 × 10–2 ± 3,91 × 10–3

8.42 × 10–3 ± 3.51 × 10–3

7.59 × 10–4 ± 1.16 × 10–4

1.63 × 10–4 ± 3.80 × 10–5

9.71 × 10–3 ± 3.20 × 10–3

1.29 × 10–3 ± 2.86 × 10–4

2.81 × 10–1 ± 1.88 × 10–1

1.32 × 10–2 ± 1.56 × 10–3

Co8

2.95 × 10–1 ± 2.84 × 10–1

7.11 × 10–3 ± 1.66 × 10–3

8.78 × 10–4 ± 4.39 × 10–4

1.92 × 10–4 ± 1.01 × 10–4

6.49 × 10–3 ± 2.61 × 10–3

1.20 × 10–3 ± 3.65 × 10–4

1.70 × 10–1 ± 1.01 × 10–1

1.24 × 10–2 ± 1.27 × 10–3

  1. Conjugation frequencies shown are the mean of three independent experiments ± standard deviation, each carried out with four biological replicates. Conjugation frequency was calculated as the number of transconjugants/(number of recipients × donor-to-recipient ratio.
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