Figure 2
From: Expression and processing of mature human frataxin after gene therapy in mice
Workflow for quantification of mature hFXN and mFXN. Covalent linking of a rabbit mAb to protein G beads facilitated efficient recovery of mFXN and hFXN proteoforms from tissues for 2D-nano-UHPLC-PRM/HRMS analysis, as it allowed the beads to be washed to remove matrix contaminants. 2D-nano-UHPLC-PRM/HRMS = two-dimensional nano-ultra-high performance liquid chromatography-parallel reaction monitoring high-resolution mass spectrometry; DMP = dimethyl pimelimidate dihydrochloride; FXN = frataxin; hFXN = human frataxin; mAb = monoclonal antibody; mFXN = mouse frataxin; SILAC = stable isotope labelling by amino acids in cell culture.
