Table 6 Results of robustness tests of analytical methods (n = 3).
Method | Conditions | Values | Average recovery % | R.S.D. % |
|---|---|---|---|---|
HPLC method | Standard method conditions | 99.99 | 0.327 | |
The high flow rate of the mobile phase | 1.10 mL min−1 | 99.67 | 0.397 | |
The low flow rate of the mobile phase | 0.90 mL min−1 | 99.52 | 0.410 | |
The high-detection wavelength | 275 nm | 99.70 | 0.231 | |
The low-detection wavelength | 271 nm | 99.75 | 0.332 | |
The high amount of ethanol in the mobile phase | 32% | 99.72 | 0.264 | |
The low amount of ethanol in the mobile phase | 28% | 99.60 | 0.309 | |
Spectrophotometric method | Standard method conditions | 99.93 | 0.394 | |
The high-detection wavelength | 275 nm | 99.53 | 0.341 | |
The low-detection wavelength | 271 nm | 99.34 | 0.453 | |
Solvent | Ethanol | 99.45 | 0.407 | |
Solvent | Isopropyl alcohol | 99.17 | 0.400 | |
