Table 1 Primer sequence, target gene, amplicon sizes and cycling conditions.

From: Efficacy evaluation of hydrogen peroxide disinfectant based zinc oxide nanoparticles against diarrhea causing Escherichia coli in ruminant animals and broiler chickens

Target

Primers sequence

Amplified segment (bp)

Primary denaturation

Amplification (35 cycles)

Final extension

Reference

Secondary denaturation

Annealing

Extension

ERIC

ATG TAA GCT CCT GGG GAT TCA C

Variable

94 °C

94 °C

52 °C

72 °C

72 °C

Versalovic et al.28

AAG TAA GTG ACT GGG GTG AGC G

5 min

30 s

1 min

1 min

10 min

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