Figure 3 | Scientific Reports

Figure 3

From: Diverse effects of a Cyperus rotundus extract on glucose uptake in myotubes and adipocytes and its suppression on adipocyte maturation

Figure 3

Effect of ECR on the expression of glucose transporters and Akt signaling protein in L6 and 3T3-L1 cells. Western blotting demonstrated the expression of p-Akt, Akt, GLUT1, GLUT4, and β-actin in L6 myotubes (a) and 3T3-L1 adipocytes (e) incubated with ECR at concentrations of 25, 50, and 100 μg/mL for 48 h. The relative protein levels of p-Akt/Akt (b, L6 cells; f, 3T3-L1 cells), GLUT1 (c, L6 cells; g, 3T3-L1 cells), and GLUT4 (d, L6 cells; h, 3T3-L1 cells) were determined using ImageJ program and normalized against β-actin level. Insulin (Ins, 100 nM) and metformin (Met, 1 mM) were used as positive controls. The error bars represented ± SEM of triplicate data. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; statistical significance compared to the control.

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