Figure 9
From: PAFAH1B3 is a KLF9 target gene that promotes proliferation and metastasis in pancreatic cancer
Correlation between KLF9 and PAFAH1B3 protein expression in pancreatic cancer tissues and cells. (A) Spearman correlation analysis of KLF9 and PAFAH1B3 in pancreatic cancer. (B) Representative images of PAFAH1B3 and KLF9 staining in PDAC tissues and adjacent normal tissues shown by IHC. (C) Protein levels of KLF9 in HPDE6-C7 cells and SW1990, MIA Paca-2 and PANC-1 cells detected via Western blotting. SW1990 and MIA Paca-2 cells were transfected with pcDNA3.1-KLF9 or pcDNA3.1 and subjected to RT‒PCR assays (D) and Western blotting assays (E). SW1990 and MIA Paca-2 cells were transfected with siKLF9 or siNC and subjected to RT‒PCR (F) and Western blotting (G). β-Actin was used as an internal control. The data represent the average of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001.
