Figure 5 | Scientific Reports

Figure 5

From: De novo reconstruction of a functional in vivo-like equine endometrium using collagen-based tissue engineering

Figure 5

(A) The progression of an in vitro 3D-ET from Day 1 to Day 7 of culture. At D1, eMSCs (red arrow) exhibit a spindle-like shape in the collagen scaffold, while eECs display a round cellular shape (yellow arrow). By the following day, eECs have undergone cell division and differentiate into gland-like structures. By Days 5–10, these glands develop into tubular structures. (B) Confocal microscopy Z-stack panoramic view of the 3D structure of the 3D-ET. (C) The z-stack showing the various layers of the 3D-ET from left to right. The top view illustrates eECs positioned at the uppermost layer of the endometrial tissue. Red arrows highlight eMSCs in each layer of the 3D-ET, displaying a three-dimensional spindle shape with elongated processes. (D) Confocal microscopy shows that these ‘glands’ were hollow.

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