Table 1 Primer pairs for conventional and real-time PCR of genes expressed by equine endometrial epithelial cells (eECs) and mesenchymal stromal cells (eMSCs) (*see Rink et al.25.
Primer name | Sequence | Accession number | Annealing temperature (°C) | Amplicon size (bp) |
|---|---|---|---|---|
BAX | F: TTT GCT TCA GGG TTT CAT CC R: ATC CTC TGC AGC TCC ATG TT | XM_001489207.1 | 53 | 197 |
BCL2 | F: GAG ACC CCC AGT GCC ATC AA R: GGG ATG TCA GGT CGC TGA AT | XM_001499714.1 | 58 | 233 |
COL1A1 | F: TGC GAA GAC ACC AAG AAC TG R: GAC TCC TGT GGT TTG GTC GT | XM_023652710.1 | 56 | 108 |
CD29* | F: GGC TAA CAG GGA GTT TCA GAT R: ACA TCT ATT TTC ATC TGC TTG GC | NM_001301217.1 | 53 | 100 |
CD44* | F: CCC ACG GAT CTG AAA CAA GTG R: TTC TGG AAT TTG AGG TCT CCG TAT | XM_023653788.1 | 58 | 95 |
CD90* | F: TGC GAA CTC CGC CTC TCT R: GCT TAT GCC CTC GCA CTT G | XM_023644995.1 | 53 | 93 |
Foxa2 | F: GTG ATT GCT GGT CGT TTG TTG R: TGT TCA TGC CGT TCA TCC C | XM_023626003.1 | 55 | 186 |
IL6 | F: CAA TCT GGG TTC AAT CAG GA R: GAA GGA TGA GGT GAG TTG TT | NM_001082496.2 | 53 | 277 |
LIMK1 | F: AGA GGA AGG AAG CGA GTT GC R: CCA TGA CCA GCC CCT TAG TG | XM_023655469.1 | 56 | 245 |
LIMK2 | F: GTT TAT CTG GTG TGG GAA GAA G R: CTG GGT GGT ACT TGA ACT CC | XM_001497153.5 | 56 | 239 |
Muc1* | F: CTA TCT CGT TGC CCT GGC TG R: GTA GGC ATC ACG GGT TGG AA | XM_023641050.1 | 58 | 82 |
RHOA | F: CGA TTC TCT CGT TGG TCC R: TCC TTG CTA AAG ACG ATG AG | XM_023619803.1 | 50 | 280 |
ROCK1 | F: ACT GGA AAA CTG ATT CGT CT R: TCA GCC ATG AGA AAA CAC C | NM_001163985.2 | 50 | 192 |
ROCK2 | F: AGA GAT TTC ACC AGC ACA TT R: CCA GGT ACA CAA ATG AGT GA | XM_023619528.1 | 51 | 300 |
PGFS | F: TGG GTT CCG CCA TAT TGA TT R: CAA CTC GGG TCG AAG GAA AG | NM_001081895.2 | 55 | 151 |
LPL | F: ACG TCT TAC ACA CAT TCA CC R: TTG GAA AGT GCC TCC ATT AG | AB278557 | 53 | 101 |
GAPDH* | F: GAA GAT GTG GCG CGA TGG CC R: ACT GAC ACG TTA GGG GTG GGG AC | NM_001163856.1 | 65 | 147 |
