Figure 5
Hypoxia resulted in decreased leptin, ob-Rb, and HIF1α protein levels in rat myocardial tissues. (A) Plasma leptin. (B) Western blot analysis of HIF1α, leptin, and Ob-Rb in left ventricular myocardial tissues. (C–E) Quantification of HIF1α, leptin, and Ob-Rb protein expression in left ventricular myocardial tissues. (F) Western blot analysis of HIF1α, leptin, and Ob-Rb in right ventricular myocardial tissues. (G–I) Quantification of HIF1α, leptin, and Ob-Rb protein expression in left ventricular myocardial tissues. The protein expression levels were normalized to those of β-Tubulin. (J,K) Immunohistochemistry of leptin and Ob-Rb in left and right ventricular myocardial tissues. n = 6. Data are presented as mean ± SEM. The p values are shown on the top of compared group by two-tailed unpaired t-test (for normally distributed data) or Mann–Whitney U test (for nonnormally distributed data).
