Figure 2
Effects of treadmill training on lung cell apoptosis in rats following SCI. (A) TUNEL staining, scale bar = 50 µm. (B) CytC immunofluorescence staining, scale bar = 20 µm. (C) Bax immunofluorescence staining, scale bar = 20 µm. (D) Bcl-2 immunofluorescence staining, scale bar = 20 µm. (E) TUNEL staining-positive cell rate, n = 3. (F) CytC-positive cell rate, n = 3. (G) Bax-positive cell rate, n = 3. (H) Bcl-2-positive cell rate, n = 3. (I) caspase 9-positive cell rate, n = 3. (J) caspase 3-positive cell rate, n = 3. (K) Immunohistochemical staining for caspase 9, scale bar = 50 µm. (L) caspase 3 immunohistochemical staining, scale bar = 50 µm. *P < 0.05, compared with the Sham group at the same time point; #P < 0.05, compared with the SCI group at the same time point; @P < 0.05, compared with the same group on day 3; ▲P < 0.05, compared with the same group on day 7. P < 0.05 was determined by two-way ANOVA (Bonferroni’s multiple comparison test).
