Figure 3
From: Optimization of plasma-based BioID identifies plasminogen as a ligand of ADAMTS13
Proteins identified from the in-vitro BioID screen of ADAMTS13 in plasma. Venn diagram of the number of proteins labelled from BioID assay of 100 nM ADAMTS13-BirA*, BirA* or PBS buffer (no protein) in citrated plasma, with 1 mM ATP added every hour, and 50 µM biotin, at 37 °C for 4 h. Labelled proteins were isolated using agarose streptavidin beads, digested with trypsin, and identified using LC–MS/MS. The numbers represent the number of unique labelled proteins present in each condition.
