Figure 4
From: Optimization of plasma-based BioID identifies plasminogen as a ligand of ADAMTS13
Binding of various analytes to immobilized ADAMTS13. Analytes Glu-Plasminogen (Glu-Pg), vitronectin (Vn), stanniocalcin-2 (STC2) and bovine serum albumin (BSA) were passed over immobilized ADAMTS13 at varying concentrations (0–7 µM). Change in response units was monitored over time, and corrected using a blank empty flow channel. Equilibrium value response units are plotted as a function of analyte concentration. Data points are presented as mean ± standard deviation or 3 biological replicates. Data points are fitted using a one-site binding model using GraphPad Prism.
