Figure 6
From: Optimization of plasma-based BioID identifies plasminogen as a ligand of ADAMTS13
Effect of EACA or TXA onto Glu-Pg and Lys-Pg binding to immobilized ADAMTS13. Increasing concentration (0–5 mM) of EACA or TXA were injected along with 2 µM Glu-Pg or Lys-Pg onto immobilized ADAMTS13. Change in response units was monitored over time, and corrected using a blank empty flow channel. Equilibrium value response units are plotted as a function of analyte concentration. Data points are presented as mean ± standard deviation or 3 biological replicates. Data points are fitted using a one-site binding model using a competitive inhibition model ([inhibitor] vs normalized response—variable slope) using GraphPad Prism (V9).
