Table 3 Matrix of simplex centroid design and results for DPPHIC50, and ABTSIC50.

From: Design of three-component essential oil extract mixture from Cymbopogon flexuosus, Carum carvi, and Acorus calamus with enhanced antioxidant activity

Experiment numbera

C. flexuosus

C. carvi

A. calamus

Observed responsesb

DPPH IC50 (µg/mL)

ABTS IC50 (µg/mL)

1

1

0

0

874.22 ± 4.13

1034.17 ± 5.87

2

0

1

0

194.1 ± 2.01

152.67 ± 3.19

3

0

0

1

349.04 ± 3.73

193.42 ± 2.52

4

0.5

0.5

0

361.67 ± 1.02

438.39 ± 0.28

5

0.5

0

0.5

517.82 ± 3.21

410.15 ± 1.14

6

0

0.5

0.5

281.21 ± 1.18

132.06 ± 2.17

7

0.33

0.33

0.33

185.34 ± 1.07

112.27 ± 0.76

8

0.33

0.33

0.33

189.2 ± 0.84

102.33 ± 1.71

9

0.33

0.33

0.33

202.15 ± 0.41

82.25 ± 1.06

10

0.67

0.17

0.17

591.21 ± 1.05

644.13 ± 0.93

11

0.17

0.67

0.17

265.32 ± 1.45

110.48 ± 3.02

12

0.17

0.17

0.67

316.46 ± 1.17

179.02 ± 1.78

BHT

   

191.13 ± 0.83

114.07 ± 1.42

  1. aExperiments were performed after randomization.
  2. bThe tests were conducted in three independent replicates and established as means ± SD.
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