Figure 2

Glial Draper required for experience-dependent critical period pruning. (A) Representative images of Or42a OSNs innervating the paired VM7 synaptic glomeruli with Or42a-Gal4 driving the membrane marker UAS-mCD8::GFP (Or42a > GFP, green) following 24-h critical period exposure to oil vehicle control (top) or 25% EB (bottom). Robust pruning of VM7 innervation occurs in the genetic background control (left, arrows) following 0–1 dpe EB odorant experience, but no pruning happens in the draper null mutant (draper^∆5, right). Scale bar: 10 µm. (B) Quantification of the innervation volume of Or42a OSNs in the VM7 glomeruli in all four genotypes, normalized to the oil vehicle control. Scatterplots show all data points and the mean ± SEM. Significance is indicated as not significant (NS; p > 0.05), or significant at p ≤ 0.0001 (****). (C) Glial-targeted draper RNAi prevents critical period experience-dependent pruning. Representative images of Or42a OSNs innervating VM7 synaptic glomeruli in glial attp2 TRiP transgenic driver controls (repo-Gal4/attP2; left) or glial draper RNAi (right) following 24-h critical period exposure to the oil vehicle (top) or 25% EB odorant (bottom). The robust pruning apparent in the controls (left, arrows) is prevented by glial-targeted draper RNAi. Scale bar: 10 µm. (D) Quantification of normalized innervation volume of Or42a OSNs in VM7 glomeruli in all four genotypes. Scatterplots show all data points and mean ± SEM. The significance is indicated as not significant (NS; p > 0.05), or significant at p ≤ 0.0001 (****).