Figure 3
From: Experience-dependent glial pruning of synaptic glomeruli during the critical period
Ensheathing glia mediate experience-dependent critical period pruning. (A) Representative images of the Or42a OSN innervation of the VM7 synaptic glomeruli (Or42a > GFP, green) with 24-h critical period exposure to oil vehicle (left) or EB (right). Robust innervation pruning occurs in Gal4 driver control (R56F03-Gal4/+), with oil vehicle (top left) and following EB experience (top right, arrows). Similar pruning occurs with both cortex glia (R54H02-Gal4) and astrocyte-like glia (R86E01-Gal4) draper RNAi (middle). In contrast, ensheathing glia (R56F03-Gal4) draper RNAi blocks experience-dependent pruning, with normal innervation in the oil vehicle (bottom left) and indistinguishable maintained innervation following 0–1 dpe EB odorant experience (bottom right). Scale bar: 10 µm. (B) Quantification of Or42a OSN innervation volume in VM7 glomeruli in all four genotypes, normalized to the driver oil vehicle control. For each glial class driver, paired 24-h critical period exposure from 0–1 dpe to oil vehicle (left) and 25% EB (right) is shown. Scatterplots show all the data points and mean ± SEM. The significance is indicated as not significant (NS; p > 0.05), or significant at p ≤ 0.0001 (****).
