Figure 4
From: Experience-dependent glial pruning of synaptic glomeruli during the critical period
Glial JNK signaling drives experience-dependent critical period pruning. (A) Representative images of Or42a OSNs innervating VM7 olfactory synaptic glomeruli (Or42a > GFP, green) following 24-h critical period exposure to the oil vehicle control (top) or EB odorant (bottom) from 0–1 dpe. Striking experience-dependent pruning of the VM7 innervation occurs in the glial TRiP driver control (repo-Gal4/attP2) following critical period EB exposure (left; arrows), which is completely blocked by glial-targeted basket knockdown (repo-Gal4 driven basket RNAi, right). Scale bar: 10 µm. (B) Quantification of Or42a OSN innervation volume in the VM7 glomerulus in all four genotypes, normalized to the oil vehicle control. Scatterplots show all the data points and mean ± SEM. The significance is indicated as not significant (NS; p > 0.05), or significant at p ≤ 0.0001 (****). (C) Glial-targeted puckered phosphatase (puc) to block basket/JNK signaling prevents critical period experience-dependent pruning. The glial driver control (repo-Gal4/attP2, left) compared to glial-targeted puc overexpression (pucOE, right) with the oil vehicle (top) and EB experience (bottom). Or42a OSN pruning in the VM7 glomeruli (left, arrows) is prevented by glial-targeted pucOE to block glial basket/JNK signaling. Scale bar: 10 µm. (D) Normalized quantification of Or42a OSN innervation volume in VM7 glomeruli in all four genotypes. Scatterplots show all data points and the mean ± SEM. The significance is indicated as either not significant (NS; p > 0.05), or significant at p ≤ 0.0001 (****).
