Figure 5
From: Experience-dependent glial pruning of synaptic glomeruli during the critical period
Critical period experience signals Basket/JNK glial nuclear translocation. (A) Odorant experience in the critical period drives the nuclear translocation of activated Basket/JNK in glia to modulate downstream transcriptional regulation. Representative high magnification images of glia immediately adjacent to the VM7 glomerulus, which are double-labeled for glial repo-Gal4 targeted UAS-basket::GFP (bsk::GFP, green; top) and anti-Repo to mark the glial nuclei (Repo, magenta; middle), with merged images shown below (white overlap, bottom). 24-h critical period exposure from 0–1 dpe to the oil vehicle alone (left column) and with the EB odorant (right). The signal colocalization in the glial nucleus (white; bottom) indicates bsk::GFP nuclear accumulation driven by EB experience in the critical period. Scale bar: 2 μm. (B) Quantification of the glial nuclear bsk::GFP fluorescence intensity normalized to the oil control, following 24-h critical period exposure from 0–1 dpe to the oil vehicle (left) or 25% EB (right). Scatterplots show all data points and mean ± SEM, with each data point representing the average from the 10 glial nuclei closest to the VM7 glomerulus. Significance is shown at p ≤ 0.0001 (****).
