Figure 6
From: Experience-dependent glial pruning of synaptic glomeruli during the critical period
Glial JNK signaling upregulates the F-actin scaffold Cheerio/FLNA. (A) Critical period EB experience dramatically upregulates glial Cheerio/FLNA expression downstream of both Draper receptor and Basket signaling in glia. Representative high magnification images of a VM7 glomerulus (dotted outlines) labeled for anti-Cheerio (red) in the glial transgenic driver control (repo-Gal4/attP2, left), glial-targeted draper RNAi (middle), and glial-targeted basket RNAi (right) following the 24-h critical period exposure from 0–1 dpe to the oil vehicle alone (top) or with the EB odorant (bottom). Experience-dependent Cheerio expression upregulation within the VM7 synaptic glomerulus (arrows) completely depends on glial Draper and Basket signaling. Scale bar: 5 μm. (B) Normalized quantification of the Cheerio fluorescence intensity expression levels in the repo-Gal4/attP2 driver control (left) compared to glial-targeted draper RNAi (middle) and basket RNAi (right), with both oil vehicle control and EB odorant exposure from 0–1 dpe. Scatterplots show all data points and the mean ± SEM. The significance is indicated as not significant (NS; p > 0.05), or significant at p ≤ 0.0001 (****).
