Figure 7
From: Experience-dependent glial pruning of synaptic glomeruli during the critical period
Cheerio remodels F-actin cytoskeleton for experience-dependent pruning. (A) Glial Cheerio is required for critical period experience-dependent pruning of olfactory sensory neuron innervation. Representative images of the Or42a OSNs innervating VM7 synaptic glomeruli (Or42a > GFP, green) following 24-h critical period exposure to the oil vehicle control (top) or EB odorant (bottom) from 0–1 dpe. Robust glial pruning of the VM7 innervation occurs in the glial transgenic driver control (repo-Gal4/attP2) following EB experience (left, arrows), which fails completely with repo-Gal4 glial-targeted cheerio RNAi (right). Scale bar: 10 μm. (B) Quantification of the Or42a OSN innervation volume in the VM7 glomeruli of all four genotypes, normalized to the oil vehicle control. Scatterplots show all data points and mean ± SEM. The significance is indicated as not significant (NS; p > 0.05), or significant at p ≤ 0.0001 (****). (C) High magnification images of glial repo-Gal4 driven F-actin marker LifeAct::GFP in VM7 glomeruli (dotted outline) following 24-h critical period exposure to oil vehicle (top left) or EB odorant (top right) from 0–1 dpe, showing the experience-dependent remodeling of the F-actin cytoskeleton. Glial-targeted draper and cheerio RNAi prevents experience-dependent F-actin induction in VM7 glomeruli (bottom images). Scale bar: 5 μm. (D) Normalized quantification of glial LifeAct::GFP fluorescence intensity levels in VM7 glomeruli with 24-h critical period exposure to the oil vehicle or 25% EB. Scatterplots show all data points and mean ± SEM. The significance following a one-way ANOVA is indicated at p ≤ 0.0001 (****).
