Figure 6
From: Dysregulated gene subnetworks in breast invasive carcinoma reveal novel tumor suppressor genes
Overexpression of NLGN3 and ANK2 restricts breast cancer proliferation and migration. I. Cell proliferation quantified by MTT assay in control and NLGN3, ANK2 overexpressed MCF7 and MDA-MB-231 cells post 48hr. II. Overexpression of NLGN3 and ANK2 restricts breast cancer proliferation and migration. Colony forming assay showing number of colonies per plate along with graphical representation. III. Migration assay showing the number of migrated cells through trans-well chamber 48h after stimulation with 5% FBS. IV. Wound-healing showing 0 hr, 12 hr, and 24 hr post wounding and its graphical representation showing percentage wound closure in ctrl, ANK2O/E and NLGN3O/E-treated MCF7 and MDA-MB-231 cells. Data are represented as mean ± SD. 2 way ANOVA was used for the statistical analyses, n = 3 (****P < 0.0001,***P < 0.001, **P < 0.01, *P < 0.05).
