Figure 8
From: Dysregulated gene subnetworks in breast invasive carcinoma reveal novel tumor suppressor genes
The flowchart of methodology. The Cancer RNA-seq Nexus (CRN) and International Cancer Genome Consortium (ICGC) were used as data sources as indicated in the figure. The differential expression analysis was performed on BRCA (breast invasive carcinoma) and NAT (Normal Adjacent Tissue) samples in three major molecular classes of BRCA in early and late stage. The genes were clustered into subnetworks based on their frequency of mutation. The pathway and functional enrichment analysis were performed on selected clusters of genes and selected genes were validated using qRT-PCR, western blot and immunohistochemistry. IPA=Ingenuity Pathway Analysis. qRT-PCR=Quantitative Real-Time Polymerase Chain Reaction. SNP=Single Nucleotide Polymorphism.
