Figure 5

Histological findings at 1 h (A,B) and 3 h (C–E) after i-PDT. (A,C) HE staining, (B,D,E) TUNEL method. Asterisk: location of the light source, Arrow: direction of fiber insertion. The degree of tissue damage caused by i-PDT did not differ at 1 and 3 h after i-PDT. In both timings, tissue necrosis was observed in the area close to the light source, and TUNEL-positive apoptosis was seen in the area a little further away from the light source.