Figure 1

Genetic diversity and phylogenetic relationships of H. japonica and H. megaspinosa specimens from Japan revealed by Bayesian MCC tree of concatenated of 15 mitochondrial gene sequences. The tree was rooted to H. flava which is the putative sister-species to H. japonica and H. megaspinosa (Barker unpublished data). In the tree, the clade representing specimens from H. megaspinosa are highlighted in blue (Hm) while H. japonica specimens were subdivided into two clades that are highlighted in light cyan (Hj1) and green (Hj2). The nucleotide differences among the 47 newly sequenced mitogenomes of Haemaphysalis collected in Japan were examined. The positions of single nucleotide variations are indicated by vertical lines in the mitogenome sequences.