Figure 6
Activity-based labeling of mouse Cyp1a2 when expressed in planta. (a) MmCyp1a2-GFP localizes to the ER when expressed in planta. MmCyp1a2-GFP was transiently co-expressed with ER marker RFP-HDEL by agroinfiltration. Confocal images were taken 5 days after agroinfiltration. Scalebar 5 μm. Experiment was repeated twice with similar results. (b) NADPH-dependent labeling of mouse MmCyp1a2 expressed in planta. Microsomes were isolated from agroinfiltrated leaves expressing MmCyp1a2-GFP and labeled for 1.5 h with or without 10 μM probe and with or without 1 mM NADPH. Labeled proteins were coupled to Cy2-picolyl-azide via click chemistry and visualized by in-gel fluorescence scanning. Western blot with anti-GFP antibodies shows the MmCyp1a2-GFP fusion accumulating at 82 kDa (arrowhead). *RBSL.
