Figure 1
From: Transcriptional repression of the oncofetal LIN28B gene by the transcription factor SOX6
Expression levels of LIN28 and SOX6 in erythropoiesis during development. (a) RT-qPCR: Sox6, Lin28B and globins expression relative to GAPDH in E11.5-E13.5 mouse fetal liver (FL) and in adult bone marrow (BM). The decline in Lin28B and the opposite increase in Sox6 parallel the transition (hemoglobin switching) from embryo/fetal (Hbb-y and Hbb-bh1) to adult (Hbb-b1/2) mouse globins. In the Figure, * = p < 0.05; ** = p < 0.01; *** = p < 0.001. Lin28B and Sox6 fold changes calculated on mean values: Lin28B fold reduction E12.5-E13.5 ≈45% p = 0.0003, Sox6 fold increase E12.5-E13.5 ≈45% p = 0.012. (b) RT-qPCR and Western Blot showing the opposite expression of LIN28B and SOX6 and human fetal γ (HBG) and adult β (HBB) globins in human fetal-like HUDEP1 and adult HUDEP2 cells. (c) Western blot showing the relative protein levels.
