Figure 3
From: Transcriptional repression of the oncofetal LIN28B gene by the transcription factor SOX6
SOX6 represses LIN28B and blocks proliferation in erythroid cells. (a) Effect of SOX6 expression on LIN28B mRNA level. SOX6: cells transduced with the SOX6 overexpressing vector; Empty Vector (EV): cells transduced with the corresponding empty vector. Fold reduction calculated on the mean values: K562 ≈92% p < 0.0001, HEL ≈54% p = 0.0009, HUDEP1 ≈60% p < 0.0001. (b) Representative Western blots and quantification of the LIN28B reduction upon SOX6 overexpression at the protein level (densitometry analysis from 3 independent Western blot experiments was performed by using the ImageJ software). Fold reduction calculated on the mean values: K562 ≈48% p = 0.03, HEL ≈40% p = 0.0281, HUDEP1 ≈63% p = 0.046. (c and d) Effects of SOX6 expression on representative Let-7 downstream targets and on cell proliferation. Empty Vector (EV), in black: cells transduced with the empty vector. SOX6, in red: cells transduced with the SOX6 expressing vector. Fold reduction of targets calculated on the mean values: K562 IGF2BP1 ≈42% p = 0.0005, K562 c-MYC ≈60% p < 0.0001, K562 CBX2 ≈60% p = 0.0058, HEL, c-MYC ≈40% p = 0.0045. HUDEP1 IGF2BP1≈39% p = 0.0006, HUDEP1 c-MYC ≈64% p = 0.0008. For all RT-PCR shown, n ≥ 4, error bars: standard error of mean. *p < 0.05; **p < 0.01; ***p < 0.001.
