Figure 1
From: Mitochondrial SIRT3 as a protective factor against cyclosporine A-induced nephrotoxicity
The expression of mitochondrial SIRT3 and apoptotic marker was affected by CsA administration on kidney tubular cells. (A) Representative blots and densitometry analyses of western blot for mitochondrial SIRT3 and cleaved caspase-3 after two different concentration of CsA injection (10 uM and 20 uM) was shown. Protein expression was normalized to HSP60 for mitochondrial SIRT3 and β-actin for whole lysate SIRT3, cytosol SIRT3 and cleaved caspase-3. (B) Representative blots for SIRT3, HSP60 and VDAC to examine cellular localization of SIRT3 expression. Each blot has been cropped from different gels; uncropped gels/blots are presented in Supplementary Fig. 2. Error bars represent standard deviation. Statistical significance was assessed using Kruskal–Wallis followed by Dunn’s multiple comparison test, where ns indicates not significant, and *p < 0.05 indicates statistical significance.
