Figure 3
From: Mitochondrial SIRT3 as a protective factor against cyclosporine A-induced nephrotoxicity
SIRT3 overexpression restored cell viability and mitochondrial SOD2 expression as well as alleviated apoptosis after CsA injury. (A) Cell viability was analyzed using the MTS assay. Error bars represent standard deviation. *p < 0.05, **p < 0.01. (B,C) Representative blots and densitometry of blots were shown for cleaved caspase-3, Bcl-2, cytochrome C and Bax. Protein expressions were normalized to β-actin. (D) Western blot analysis was performed for SIRT3 and SOD2 in mitochondrial fraction. The representative blots of independent experiments are shown on left, and the densitometry of these blots are shown on right. Protein expressions were normalized to HSP60. *p < 0.05, **p < 0.01 (E) A representative western blot (left) and densitometry (right) of AMPKα, p-AMPKα and PGC1α expression in whole lysate. Protein expressions of PGC1α were normalized to β-actin, and p-AMPKα to total AMPKα. (F) Representative histogram of MitoSOX fluorescence intensity (upper) and quantitated total superoxide levels plotted on a bar chart (lower). Each blot has been cropped from different gels; uncropped gels/blots are presented in Supplementary Figs. 4 and 5. Significance levels marked as *p < 0.05, **p < 0.01, ****p < 0.0001 based on Kruskal–Wallis followed by Dunn’s multiple comparison test, from three independent experiments.
