Figure 5

Molecular identification of Plasmodium spp.: (a) Gel representation of 160 bp cDNA fragments of first round amplification; (b) 110 bp cDNA fragments of second round amplification. [Lane L: 100 base pair (bp) plus DNA ladder, Lane C + : positive control, Lane A1–A5: female Anopheles mosquito PCR products of first reaction of nest-PCR, Lane N1–N4: PCR products of Plasmodium species specific primers (for vivax [N1], falciparum [N2], ovale [N3] and malariae [N4]), Lane C-: negative control, Lane B: blank].