Figure 4
From: Twist2 contributes to skin regeneration and hair follicle formation in mouse fetuses
Two-dimensional (2D) and three-dimensional (3D) culture of dermal fibroblasts and observation of their ability to undifferentiate. (A) 2D and 3D culture of fibroblasts derived from the superficial and deep dermis layers on E17. S, superficial layer; D, deep layer. (B) qPCR analysis of the gene expression of Twist2 and undifferentiated markers. RQ, relative quantification. *P < 0.05. (C) qPCR analysis of the gene expression of undifferentiated markers after Twist2 knockdown. *P < 0.05. (D) qPCR analysis of hair induction-related gene expression. RQ, relative quantification; qPCR, quantitative polymerase chain reaction; Neg siRNA, negative control siRNA. *P < 0.05.
