Figure 3

Apoptosis associated p53 downstream genes were regulated by CUL4B. (A) RT-qPCR analysis of  Puma and Bax expression in Cul4b^WT and Cul4b^ΔIEC mice before and after radiation at 3 days. Error bars represent SD, *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001, based on Student’s t test. (B) RT-qPCR analysis of Puma and Bax expression in shCul4b and shNC IEC-6 cells before and after radiation at 24 h. Error bars represent SD, *P < 0.05; **P < 0.01; ****P < 0.0001, based on Student’s t test. (C, D) Western blot and statistical analysis of CUL4B and BAX expression in intestine from Cul4b^WT and Cul4b^ΔIEC mice before and after radiation at 3 days. Error bars represent SD, ***P < 0.001; ****P < 0.0001, based on Student’s t test. (E, F) Western blot and statistical analysis of CUL4B and BAX expression in shCul4b and shNC IEC-6 cells before and after radiation at 24 h. Error bars represent SD, *P < 0.05; **P < 0.01, based on Student’s t test. The original western blots are presented in Fig. S6.