Figure 4

PSME3 bridged CUL4B and its regulation of p53. (A) Venn diagram of CUL4B binding-protein using MS in intestine of Cul4b^WT and Cul4b^ΔIEC mice pre-IR and post-IR at 3 days. (B) Venn diagram of the overlap of up-regulated ubiquitylated proteins and down-regulated proteins identified in Cul4b^ΔIEC mice compared with Cul4b^WT mice. (C) Changing folds and detail modification site of PSME3. (D, E) Western blot and statistical analysis of the expression of p53 in Psme3-knockdown (siPsme3) and PSME3-overexpressed (OE PSME3) IEC-6 cells relative to control (siNC and OE NC). Error bars represent SD, **P < 0.01; ***P < 0.001; ****P < 0.0001, based on Student’s t test. The original western blots are presented in Fig. S6.