Figure 2
Naloxone-precipitated somatic withdrawal signs in acute and chronic morphine-administered mice (A,B) Schematic representation of experimental timelines of naloxone-precipitated withdrawal sign scoring for acute and chronic morphine-treated mice, respectively. (C,E) Increased global withdrawal sign score after naloxone injection in acute and chronic morphine treated mice, respectively. (D,F) Withdrawal sign scoring in 5-min time bins after naloxone injection in acute (D) and chronic (F) morphine treated mice. (G,I) Decreased horizontal activity score after naloxone injection in acute and chronic morphine treated mice, respectively. (H,J) Increased number of jumping events after naloxone injection in acute and chronic morphine treated mice, respectively. (K,M) Increased number of paw tremors after naloxone injection in acute and chronic morphine treated mice, respectively. (L,N) Ptosis score after naloxone injection is unaltered in acute (L) and increased in chronic (N) morphine treated mice. (O,Q) Number of teeth chattering events after naloxone injection is unaltered in acute (O) and increased in chronic (Q) morphine treated mice. (P,R) Piloerection score is not affected after naloxone injection in acute (P) and is increased in chronic morphine (R) treated mice. (S,U) Number of sniffing events is not affected after naloxone injection in acute (S) and is increased in chronic morphine (U) treated mice. (T,V) Number of head shakes is increased after naloxone injection in acute (T) but not in chronic morphine (V) treated mice. Data are represented as mean ± SEM. Dots represent individual values. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. N = 10–15/group.
