Figure 1
From: Development of a novel high-throughput culture system for hypoxic 3D hydrogel cell culture
Scheme of the high-throughput cell culture device Oli-Up. The system consists of (a) mounts with 6 scaffold holders (dimensions are given in mm). The scaffold holders are manufactured in the shape of a truncated cone which can be loaded with 15 µl hydrogel. (b) The scaffold mounts are placed upside down to enable accurate pipetting of the hydrogel into the scaffold holders. (c) Up to 8 mounts with each 6 scaffold holders can be placed in a conventional 48-well plate for crosslinking of the hydrogel and addition of medium. (d) The Oli-Up system is closed with a modified lid for culture incubation.
