Figure 3
From: Development of a novel high-throughput culture system for hypoxic 3D hydrogel cell culture
The HRE expression is dependent on the cell density and oxygen concentration. (a) Representative micrographs and (b) quantitative read-out of the hypoxia-responsive HRE-reporter MSCs encapsulated in GelMA at different seeding densities and oxygen concentrations, measured in a 96-well plate. HRE expression at (c) 5% O2 and at (d) 1 × 106 c/mL reveals that HRE expression is increased at higher cell densities and lower oxygen concentrations (n = 3).
