Figure 4
From: Development of a novel high-throughput culture system for hypoxic 3D hydrogel cell culture
Cell-specific oxygen consumption rate (OCR) of GelMA-encapsulated and 2D MSCs. (a) Cell-specific OCR of MSCs in 2D culture and GelMA hydrogels at 21 or 5% O2 (n = 3) indicates primarily that OCR levels in both 2D and 3D are highly comparable. (b) Cell-specific OCR of MSCs in GelMA at different seeding densities (n = 3) shows that the OCR is dependent on the cell density in normoxia (21% O2) but not in hypoxia (5% O2) and that the OCR is significantly lower in hypoxic conditions.
