Figure 1 | Scientific Reports

Figure 1

From: Regulation of cytokine and chemokine expression by histone lysine methyltransferase MLL1 in rheumatoid arthritis synovial fibroblasts

Figure 1

Enhanced expression of mixed-lineage leukemia 1 (MLL1) after tumor necrosis factor α (TNFα) stimulation in rheumatoid arthritis (RA) synovial fibroblasts (SFs) compared to osteoarthritis (OA) SFs. (A) MLL1 mRNA levels in OASFs and RASFs at various time points (0, 8, 16, 24, and 48 h) after stimulation with 10 ng/ml TNFα, as determined by quantitative reverse transcriptase (RT) − polymerase chain reaction (PCR). Bars show the mean ± standard error of the mean (SEM) (n = 7 OA patients and 7 RA patients). Values are expressed as the fold-increase versus the value for OASFs at 0 h. (B) Representative MLL1 protein expression patterns in SFs from 3 OA patients and 3 RA patients by western blotting analysis. Vinculin was used as the internal control. Original blots are presented in Supplementary Figs. 1A and B. (C) The quantified data of MLL1 protein levels are presented as mean ± SEM (n = 7 OA patients and 7 RA patients). The intensity of MLL1 was normalized based on Vinculin. * = P < 0.05; ** = P < 0.01 by Mann–Whitney U test.

Back to article page