Figure 4 | Scientific Reports

Figure 4

From: Regulation of cytokine and chemokine expression by histone lysine methyltransferase MLL1 in rheumatoid arthritis synovial fibroblasts

Figure 4

High levels of H3K4me3 that are induced by MLL1 in the promoters of cytokine and chemokine genes in RASFs. (A) Levels of H3K4me3 in the IL-6, IL-15, CCL2, CCL5, CXCL9, CXCL10, CXCL11, and CX3CL1 promoters in OASFs and RASFs, as determined by quantitative ChIP-PCR. Bars show the mean ± SEM (n = 8 OA patients and 14 RA patients). H3K4me3 levels are normalized by input DNA and are expressed as %INPUT. * = P < 0.05; ** = P < 0.01; *** = P < 0.001 by Mann–Whitney U test. (B) A scheme for regulation of cytokine and chemokine gene expression by MLL1-mediated H3K4me3 in OASFs and RASFs. Highly expressed MLL1 leads to cytokine and chemokine gene activation by increasing H3K4me3 levels in RASFs compared with OASFs. See Figs. 1, 2, and 3 for other definitions.

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