Figure 3
Example of temporal changes in engrafted hiPSC-RPE strips (M4). (A) Schematic diagram of the hiPSC-RPE strip transplantation site. RPE-damaged site created by the microsecond pulse laser treatment. hiPSC-RPE strip cells were transplanted at both the RPE-damaged and intact sites. (B) Image of the hiPSC-RPE strip. (C) Engraftment of the hiPSC-RPE strip at the intact site of the monkey eye. Expansion of hiPSC-RPE strips was observed by both color fundus and autofluorescence images. (D) Engraftment of hiPSC-RPE strips at the RPE-damaged site of the monkey eye. Human iPSC-RPE strips expanded from the early observation time point in both color fundus and autofluorescent images. Blue arrows indicate transplanted hiPSC-RPE, and the white arrows indicate the sites of microsecond pulse laser treatment. (E) Optical coherence tomography (OCT) images after iPSC-RPE strip transplantation to the RPE-damaged site. (F) Fluorescent retinal angiography examination after iPSC-RPE strip transplantation to the RPE-damaged site. The yellow dotted line shows the site of the microsecond pulse laser treatment and hiPSC-RPE strips expanded and block choroidal fluorescence. (G)Temporal change in the ONL thickness at the laser ablation site with or without transplantation. ONL thickness for both the transplant and non-transplant areas within laser injury site was measured at three locations (yellow bars) in the OCT images of the corresponding section throughout the observation period. The ONL thickness of the transplant site was shown as a mean ± sd ratio to that of the laser-only non-transplanted area. At all-time points, there was no significant difference in ONL thickness between the laser-only and implanted sites. ONL outer nuclear layer, INL inner nuclear layer.
