Figure 1
From: Melting temperature mapping method using imperfect-match linear long probes
Workflow of the melting temperature mapping method using imperfect-match linear long quenching probes (IMLL Q-probes). (a) Workflow of the Tm mapping method using IMLL Q-probes for identifying unknown pathogenic bacteria within three hours of whole blood collection. (b) The strategy for the primer designs is shown. Nested PCR is performed using five bacterial universal primer sets, seven IMLL Q-probes are additionally used for PCR amplicons respectively, and then seven Tm values are obtained. (c) Binding sites of each IMLL Q-probes.
