Figure 3
From: The stress-responsive cytotoxic effect of diesel exhaust particles on lymphatic endothelial cells
ISR-related genes up-regulation and cytotoxicity by intracellular ROS generated by exposure to DEPs. (A) Following DEP exposure, intracellular ROS was detected using a ROS Assay Kit. Green dots represent fluorescence colored by reacting ROS. Scale bars: 100 μm. (B) Cells were analyzed via flow cytometry following ROS exposure to DEPs. (C) DEP-induced cytotoxicity was measured in either the presence or absence of N-acetylcysteine (NAC). A Student’s t-test was performed to compare − NAC and + NAC. **p < 0.01 (n = 3). (D) The expressions of ISR-related genes were quantified via qRT-PCR in either the presence or absence of NAC. A Student’s t-test was performed to compare − NAC and + NAC. **p < 0.01 (n = 3). Data are reported as the mean ± SD.
