Figure 1 | Scientific Reports

Figure 1

From: Amperometry approach curve profiling to understand the regulatory mechanisms governing the concentration of intestinal extracellular serotonin

Figure 1

Electroanalytical performance of the boron-doped diamond (BDD) microelectrode for detection of 5-HT release from intestinal tissue. (A) Differential pulse voltammogram from distal colon mucosal tissue, showing the presence of two oxidation peaks, which corresponded to 5-HT and melatonin. Amperometric recordings at + 650 mV vs Ag|AgCl, provide selective detection of 5-HT overflow (see dotted line). (B) Stability of the BDD microelectrode for measurement of 1 and 10 µM 5-HT in Krebs buffer (n = 6). (C) Calibration plot of 5-HT on the BDD electrode (r = 0.99, n = 8).

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